T4 DNA Polymerase, 5u/ul - 100U size:100U First- strand synthesis reactions were

First- strand synthesis reactions were assembled according to manufacturer´s specifications

cloni EXPRESS BL21(DE3)

The silicabased membrane selectively binds RNA while proteins and other metabolites can be removed

Target Transcript (Size) 3´/5´ Ratios Epicentre MMLV High Performance Reverse Transcriptase Competitor I (RNase H-Mutant of MMLV RT) Competitor P (MMLV RT) ACTB (1

200 ng of EcoR I-linearized pUC19 dsDNA and 1 µg of a 100-mer ssDNA oligo were mixed in 1X TA Buffer and incubated at 37°C for 20 min in the absence or presence of 10 units of Exonuclease I

T4 DNA Polymerase, 5u/ul - 100U size:100U First- strand synthesis reactions were