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The medium in the basolateral chamber was collected at different time points and fluorescence signals were recorded using a microplate reader (FLUOstar Omega, BMG Labtech, Ortenberg, Germany) at excitation/emission wavelengths of 480 nm/530 nm, respectively

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in particular astrocyte-selective expression of mutated huntingtin with 160 GAG repeats triggered profound down-regulation of EAAT2 and HD-like phenotype in mice