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Slides were de-paraffinized in two successive baths of xylene and rehydrated in gradually decreasing ethanol baths (95%, 80%, 70% and 50%) for 5 min each, followed by immersion in distilled water

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An overexpression of GPx3 in cumulus cells may indicate a poor implantation of the embryo [23], because the GPx3 overexpression is connected with the production of ROS in oocytes and cumulus cells [13]