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The hiPSC cell lines were verified for the expression of pluripotency related genes by immunofluorescence, FACS, karyotyping and RT-qPCR 11

Analyses based on single-cell RNA sequencing and spatial transcriptome data have also revealed higher abundances of M1-type macrophages in the wound skin tissues of healed DFU patients, while non-healed patients show higher abundances of M2-type macrophages [38]

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This step is crucial to prevent the co-separation of free drug with liposomes or the premature leakage of entrapped drug, which are common sources of error in determining encapsulation efficiency of liposomes