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HCD fragmentation was performed in the HCD cell, with the readout in the Orbitrap mass analyser at a resolution of 15,000 (isolation window of 1.4 Th) and an AGC target value of 1 10 5 with a maximum injection time of 25 ms and a normalised collision energy of 27%

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The water molecules themselves can participate in reactions (like hydrolysis) that break peptide bonds

Proper handling during storage helps reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation that may affect experimental consistency

10.1016/j.freeradbiomed.2019.04.008 72 Villas-BasJ

Breakfast, lunch or dinner all work, stick with the time that fits your routine