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Blood samples were collected according to a standardised operating procedure using 0.109 M buffered sodium citrate for the CTC preparation, BD Vacutainer tubes containing K 2 EDTA for plasma samples and BD Vacutainer tubes containing clot activator and gel separation for serum samples

PCOS Group: Received subcutaneous injections of DHEA (6 mg/100 g body weight dissolved in 0.2 ml sesame oil) for 21 days to induce PCOS, followed by no additional treatment for 30 days

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Increased adiposity is linked to decreased lung function possibly with asthma-like obstructive changes or restrictive deficits due to thoracic cage impedance [82, 83]

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The total levels of glutathione (GSH + GSSG) in plasma were quantified using the Glutathione Colorimetric Detection Kit (Invitrogen, Catalog Number: EIAGSHC)