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After injection, oocytes were arrested for 18 h in M2 medium containing 2.5 M milrinone (S2484, Selleck) to allow complete degradation of Pcbp1 mRNA

Unlike disulfide linkers, which rely on intracellular glutathione (GSH) levels, acid cleavable linkers are entirely triggered by acidic environments, making them less dependent on variable intracellular redox states

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Glutathione is a Master Antioxidant & Detoxifier

Liquid chromatography tandem mass spectrometry (LCMS/MS) analysis Label-free proteomics was performed as described previously with minor adaptations 24

In addition, the GSH:GSSG ratio is critical for creating cellular compartments with unique redox characteristics