Dual-luciferase reporter assay Luciferase assays were performed using the Dual-Glo TM Luciferase Assay System (Promega, Madison, WI, USA), and luminescence was quantified on a GloMax 20/20 Luminometer (Promega) following the manufacturers guidelines
While the samples treated with Zn (0.5 g L 1 ) x GSH (0.4 g L 1 ), Zn (0 g L 1 ) x GSH (0.4 g L 1 ) and Zn (1 g L 1 ) x GSH (0.2 g L 1 ) give GTS percentage 69.44%, 72.22% and 75%, respectively
Los suplementos no sustituyen una alimentacin balanceada ni tratamientos mdicos especializados
109 It remains unclear whether the protective effect of UAMC-3203 in the iron/APAP model is due to reduced LPO alone or its combination with the effect on Sab
406,407 Detailed analysis of astrocytic morphology using genetic reports (either ALDH1L1-eGFP reporter mice or viral transfection with astrocyte-targeted mCherry) revealed significant decrease of astrocytic complexity and shrinkage of astrocytic territorial domains in prefrontal cortex (Fig
1c or the monothiol mechanism (i) from Fig