2.4 TMT proteomics analysis Liquid chromatography was performed on a Thermo nLC1200 in single-pump trapping mode with a Thermo PepMap RSLC C18 EASY-spray column (2 m, 100 , 75 m 25 cm) and a Pepmap C18 trap column (3 m, 100 , 75 m 20 mm)
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