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These results suggest that GGCT knockdown can significantly inhibit tumor growth and reduce GSH production and that the addition of the ROS inhibitor NAC can significantly rescue this effect

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Both diets were obtained from Research Diets (New Brunswick, NJ)

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As a positive control for apoptosis, tamoxifen (50 M) was included ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells