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Contraindications Given the limited safety data, conservative contraindications would include pregnancy or plans to become pregnant, breastfeeding, age under 18, severe liver or kidney disease, and any condition requiring medications with unknown interaction potential

LEfSe is known to reduce false positives and can highlight biological consistency across subclasses, while the KruskalWallis test used alone can achieve a lower false-negative rate compared to LEfSe 38

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