sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
[1] [4] Healthcare providers should consider alternative therapies with established safety profiles in pregnant or lactating patients whenever possible, and if glutathione injection is deemed necessary, close monitoring of both mother and child is recommended throughout treatment
Phytopathology 112, 452459
doi: 10.1128/spectrum.02152-23 205 KontoyiannisD
Although the therapeutic outcome in various genotypes is related to pharmaceutical variety, the findings demonstrate that the ACE gene may generate varied antidepressant effects
May MJ, Vernoux T, Leaver CJ, Van Montagu M, Inze D (1998) Glutathione homeostasis in plants: implications for environmental sensing and plant development
