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Interestingly, the DNA damage repair protein RRM2, often upregulated in NB to support oncogenicity, was upregulated across all conditions, and phosphorylation at the Ser-80 and Ser-86 sites was significantly downregulated by both combinations ( Supplementary Table S1 ) ( Based on the phosphoproteomic profiling we then inferred the upstream kinases responsible for the differential phosphorylation observed in each treatment versus control, using a kinase enrichment analysis ( Figure 6 )
All of it the fear I grew up with, and the strength I found in the Army to finally stand on my own ground taught me the same lesson from two different directions: how precious it is to feel safe
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However, the requirement for HSL phosphorylation has recently been challenged as studies by the Granneman group elegantly show that direct activation of PNPLA2 in the absence of PKA stimulation is sensitive to HSL inhibition, suggesting that PNPLA2-mediated generation of DAG is sufficient to promote HSL activity (49)
