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ex Fr.) Karst.) polysaccharides are used to reduce kaolin intake and Fos expression in mice and alleviate cisplatin-induced vomiting (Yan et al., 2009)

To determine the total amount of CD25 and CD69 (cell surface plus intracellular), cells were fixed with 1.5% PFA and permeabilized with FWS (FW containing 0.1% saponin) prior to antibody staining