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The N-terminal amino group of glycine, the imidazole side chain of histidine, and the deprotonated amide nitrogen of the Gly-His peptide bond together coordinate the copper(II) ion in a square-planar (or square-pyramidal with axial water) geometry

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

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