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The protein concentration of cleared supernatants was analysed by using a bicinchoninic acid assay kit (BCA, Thermo Fisher Scientific, Waltham, MA, United States)

They have: A special mix of ingredients that make vitamin B12 more available to the body

The incorporation of cystine through system Xc on the plasma membrane results in a subsequent increase in intracellular cysteine, a precursor of GSH (Figure 2)

Alcocer-Gomez, E

If the phenotypes of the mutants can be successfully restored to wild type, this will confirm that only the correct genes have been deleted and further validate the specific phenotypes ascribed to each GDH mutant

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