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Briefly, 10 L of CCK-8 reagent (Biosharp, Hefei, China) was added to each well and then the 96-well plate was continuously incubated at 37 C for 1 h

And, indeed, as you can see at 4:22 in my video , over the course of a day, significantly fewer chlorogenic acids made it into peoples bloodstreams when they drank their coffee with milk as compared to black

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