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MT-6036) was added to each flask, and the cells were incubated for 1 hour at 37C in a humidified atmosphere containing 5% CO 2

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The authors concluded that melatonin treatment reduced proinflammatory cytokines in tracheobronchial aspirate, serum nitrite/nitare levels, and improved outcome because of its antioxidative actions 130 ,131

To identify glutathione-overproducing mutants, acrolein was used as a selective agent, given glutathiones key role in protecting cells from acrolein-induced toxicity (Mano et al., 2009

Genetic variants can impact how well you produce glutathione and detoxify certain toxicants