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Recordings were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

Cells age due in large part to oxidative stress

The sorting system is time and cost-saving and widely applicable to both adherent cells and suspension cells

DO NOT USE IF NECK SEAL IS BROKEN OR MISSING

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doi: 10.1007/s12098-012-0834-5