Total protein was extracted from the liver using RIPA buffer supplemented with phenylmethanesulfonylfluoride (PMSF, 1 mM)
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However, it was clearly demonstrated that SCPG is further processed through the mercapturic acid pathway to the corresponding N-acetylcysteine conjugate [ N -acetyl- S -( p -chlorophenylcarbamoyl)cysteine, NACC], which possesses comparable anticancer activity to the parent compound
doi: 10.1080/15548627.2020.1761651 36 XuJLYuanLTangYCXuZYXuHDChengXDet al
