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Viability assay In 96-Well microplate, MCF-7 cells were incubated with various concentrations of PLGA nanoparticles (4500 g/mL) either empty (control) or encapsulating DDA-GSH-AuNCs (gold content of 0.79% w/w of PLGA) in Dulbeccos Modified Eagles Medium (DMEM) with 10% FBS for 24 hours

The liver plays a central role in energy regulation, metabolism, hormonal balance, and recovery

Samples were pulse-centrifuged, and the bead-free liquid was transferred to new low-binding Eppendorf tubes

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Among 38 identified hits, we found a subset of chloroacetamides with broad senolytic activity

The right type depends on the patients health, how severe their deficiency is, and the doctors advice