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Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.83.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol

EAAC1 is expressed in retinal neurons including RGCs, while GLAST is mainly expressed in Mller glial cells

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Since the enzyme preparation required the GSH-peroxide system for its catalytic activity, it was termed GPx