J., Rhodes, J., Locke, W., & Sax, H
LET7 TPR (450 bp, from 1 to 450) and LET7 PPase (1,002 bp, from 451 to 1452) were amplified via PCR using pHBT -p35S :: LET7-HA as a template and cloned into pHBT -p35S :: HA / FLAG and pMDC32 -p2x35S :: HA / FLAG vectors to obtain pHBT -p35S :: LET7 TPR / LET7 PPase -HA / FLAG and pMDC32 -p2x35S :: LET7 TPR / LET7 PPase -HA / FLAG
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found in their study that in clear cell renal cell carcinoma (ccRCC) tumor cells, endogenous Gln metabolism might reduce the anti-tumor effectiveness of T cells by promoting the Treg cell response (41)
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