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Purified recombinant protein (3 g) was resolved using 18% w/v SDS-PAGE in reduced (+-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250

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Studies have demonstrated that Chinese herbal medicine has the ability to significantly promote growth performance, improve blood chemical indicators, exhibit anti-inflammatory qualities, prevent damage, and boost antioxidant capacity (40)

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Small pieces of rectal tissue were also collected and stored in paraffin for later analysis

At all times, use clean tools and take careful measures