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Dai, H., et al

However, the independent 2021 replication by Sun et al

B.ZhengS

The gradient elution program was set as follows: 07 min (030% B), 79 min (3080% B), 911 min (80100% B), 1113 min (100% B), followed by re-equilibration to initial conditions (14 min, 0% B), with a constant flow rate of 0.5 mL/min