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Caspase-3 activity assay Both hepatocytes and liver tissues were lysed on ice with lysis buffer containing protease inhibitors, and the proteins were collected

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A 2016 clinical trial showed that at 2 months post-treatment, only 18.7% of patients maintained effect versus 12.5% in the placebo group

After serial dehydration and vitrification, the tissues were cut into 5-mm sections and subjected to hematoxylin and eosin (H&E) staining

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