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2016;11(9):e0162590

Inflammation was assessed by evans blue plasma extravasation technique and by histology

To provide further mechanistic insight into the role of IGF-1/IGF-1R in NK cell development, we investigated the levels of IRS-1 in human CD34 + cells cultured with either IL-15 or the combination of IL-15 and IGF-1 at 14 or 21 days and compared it with the levels of IRS-1 in 293A cells, which usually expressed normal levels of IRS-1

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10 ul of CellTiter AQueousOne Solution in 100ul fresh culture medium was added to the wells before a 13 hour incubation at 37C

Subcutaneous injection near the injury site is common for musculoskeletal issues, allowing high local concentrations while still providing systemic benefits