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These phase I oxidative reactions metabolise 210% of paracetamol to a reactive intermediate, N -acetyl- p -benzoquinone imine (NAPQI) which mediates the hepatotoxic effect of paracetamol

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Running viability, cytotoxicity, and apoptosis as separate assays on separate plates means more sample, more time and more variability between measurements

It also helps avoid any bad side effects

Enzymes like NADPH oxidases, xanthine oxidases, cytochrome P450 and other oxidases also produce ROS