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In in vitro maturation (IVM) cultures, the addition of Necrostatin-1 significantly increased the expression of GDF9, Mad2, and Bcl-2 in immature oocytes in mice and prevented oxidative stress-mediated deterioration of oocyte quality (Jo et al., 2015)

Full IGF-1 receptor density recovery requires 68 weeks after a 46 week daily dosing cycle, with receptor gene expression rebounding first (23 weeks) followed by restoration of cell surface receptor density and post-receptor signaling sensitivity (46 weeks total)

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