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Pre-fixation was done with 2.5% glutaraldehyde phosphate (0.1 M, pH 7.4) overnight at 4 C

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In physical dependence assay (isoniazid challenge assessed at 6, 14, 42 and 72 h after conditioning medication), when compared to diazepam non-conditioned healthy mice, in diazepam conditioned mice residual anticonvulsive activity was not present already at the earliest post-conditioning interval (i.e., not different latency to isoniazid-convulsions), whereas shorter preconvulsive latencies (as physical dependence/withdrawal hallmark) were noted in diazepam conditioned mice following isoniazid challenge at 42 h and at 72 h after end of conditioning treatment

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Post-transplant Healing and Hair Density Improvement PRP treatment also plays a crucial role in post-transplant healing